Identification of metabolites in Arabidopsis thaliana
نویسندگان
چکیده
Identification of Arabidopsis thaliana Col.0 metabolites by use of NMR spectroscopy is described in this chapter. Among the different extraction solvent tested, MeOD was the best solvent to extract phenolic compounds from Arabidopsis. By column chromatography using Sephadex LH-20 and prep HPLC, several flavonoids were isolated from the methanol extract of Arabidopsis, and their structures were identified by LC-MS and NMR spectroscopy as kaempferol 3-O-glucopyranoside-7-Orhamnopyranoside, kaempferol 3-O-rhamnosyl (1–2) glucoside-7-Orhamnopyranoside, kaempferol 3,7-Odirhamnopyranoside and quercetine 3-Orhamnopyranoside. Twenty four major metabolites of Arabidopsis thaliana Col.0 including amino acids, organic acids, sugars, phenylpropanoids, and flavonoids were identified and their NMR cheracteristics are also summerized in this study.
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